Preparative Separation of Acylglycerol by Centrifugal Partition Chromatography (CPC)
نویسندگان
چکیده
منابع مشابه
Centrifugal partition chromatography (CPC): Emerging separation and purification technique for lipid and related compounds
Centrifugal partition chromatography (CPC), pioneered by Ito in 1964, is a liquid–liquid chromatography without a sorbent, requiring two immiscible solvent phases. This is basically an outgrowth of countercurrent distribution, as developed by Craig and Post (1). In the most distinct variant of CPC, one liquid phase remains stationary while the second solvent phase passes through the stationary ...
متن کاملEfficient, Baseline Separation of Pyrethrins by Centrifugal Partition Chromatography
Pyrethrins are natural pesticides present in the oil extracted from the flowers of Chrysanthemum cinerariaefolium. They are potent ion-channel neurotoxins which have higher selective toxicity for insects compared to mammals. These phytochemicals have an exceptionally safe environmental profile and are an attractive natural alternative to organophosphate insecticides currently used in agricultur...
متن کاملPreparative isolation and purification of geniposide from gardenia fruits by centrifugal partition chromatography.
The iridoid glycoside, geniposide was purified by centrifugal partition chromatography (CPC) with a two-phase solvent system composed of ethyl acetate:isopropanol:water (3:2:5, v/v) from an 80% methanolic extract of fruits of Gardenia jasminoides. Preparative CPC yielded 56.2 mg of geniposide in a one-step separation of 500 mg of extract, with a purity of 95% as determined by HPLC. Isolated gen...
متن کاملLarge-scale separation of clavine alkaloids from Ipomoea muricata by pH-zone-refining centrifugal partition chromatography.
Centrifugal partition chromatography in the pH-zone-refining mode was successfully applied to the separation of alkaloids, directly from a crude extract of Ipomoea muricata. The experiment was performed with a two-phase solvent system composed of methyl tert-butyl ether (MtBE)-acetonitrile-water (4:1:5, v/v) where triethylamine (10 mM) was added to the upper organic stationary phase as a retain...
متن کاملAnion-exchange displacement centrifugal partition chromatography.
Ion-exchange displacement chromatography has been adapted to centrifugal partition chromatography. The use of an ionic liquid, benzalkonium chloride, as a strong anion-exchanger has proven to be efficient for the preparative separation of phenolic acid regioisomers. Multigram quantities of a mixture of three hydroxycinnamic acid isomers were separated using iodide as a displacer. The displaceme...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Japan Oil Chemists' Society
سال: 1992
ISSN: 1884-2003
DOI: 10.5650/jos1956.41.23